applications of electroporation of adherent cellsIn Situ, on a partly conductive slide
Identifieur interne : 000075 ( Main/Exploration ); précédent : 000074; suivant : 000076applications of electroporation of adherent cellsIn Situ, on a partly conductive slide
Auteurs : RBID : ISTEX:12033_1995_Article_BF02921607.pdfEnglish descriptors
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Abstract
Nontraumatic, simple, and reproducible procedures for the introduction of nonpermeant molecules into adherent mammalian cells byin situ electroporation are described. Ctells are grown on a glass slide, half of which is coated with electrically conductive, optically transparent, indium-tin oxide. An electric pulse is applied in the presence of the molecules to be introduced, and their effect on the cellular phenotype can be observed. The cells growing on the nonconductive side of the slide do not receive any pulse and serve as controls. Careful adjustment of electric field strength can achieve the introduction of the molecules into essentially 100% of the cells, and this treatment causes no detectable disruption to cellular metabolism. This is applied in the presence of the fluorescent dye, Lucifer yellow, causing its penetration into the cells growing on the conductive half of the slide. The migration of the dye to the nonelectroporated cells growing on the nonconductive area is microscopically observed under fluorescence illumination.
DOI: 10.1007/BF02921607
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Brownell</name><affiliation wicri:level="1"><mods:affiliation>Department of Microbiology and Immunology, Queen’s University, K7L3N6, Kingston, Ontario, Canada</mods:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Microbiology and Immunology, Queen’s University, K7L3N6, Kingston, Ontario</wicri:regionArea><wicri:noRegion>Ontario</wicri:noRegion></affiliation></author><author><name>Stanley K. W. Liu</name><affiliation wicri:level="1"><mods:affiliation>Department of Microbiology and Immunology, Queen’s University, K7L3N6, Kingston, Ontario, Canada</mods:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Microbiology and Immunology, Queen’s University, K7L3N6, Kingston, Ontario</wicri:regionArea><wicri:noRegion>Ontario</wicri:noRegion></affiliation></author><author><name>Kevin L. Firth</name><affiliation wicri:level="1"><mods:affiliation>Anatomy and Cell Biology, Queen’s University, K7L3N6, Kingston, Ontario, Canada</mods:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Anatomy and Cell Biology, Queen’s University, K7L3N6, Kingston, Ontario</wicri:regionArea><wicri:noRegion>Ontario</wicri:noRegion></affiliation></author><author><name>Leslie W. MacKenzie</name><affiliation wicri:level="1"><mods:affiliation>Ask Science Products Inc., Kingston, Ont., Canada</mods:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Ask Science Products Inc., Kingston, Ont.</wicri:regionArea><wicri:noRegion>Ont.</wicri:noRegion></affiliation></author><author><name>Charles D. Stiles</name><affiliation><mods:affiliation>Division of Cellular and Molecular Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA, </mods:affiliation><wicri:noCountry code="subField"></wicri:noCountry></affiliation></author><author><name>John A. Alberta</name><affiliation><mods:affiliation>Division of Cellular and Molecular Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA, </mods:affiliation><wicri:noCountry code="subField"></wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="RBID">ISTEX:12033_1995_Article_BF02921607.pdf</idno><date when="1995">1995</date><idno type="doi">10.1007/BF02921607</idno><idno type="wicri:Area/Main/Corpus">000052</idno><idno type="wicri:Area/Main/Curation">000052</idno><idno type="wicri:Area/Main/Exploration">000075</idno></publicationStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adherent cells</term><term>Electroporation</term><term>Gap junctions</term><term>Indium-tin oxide</term><term>Intercellular communication</term><term>Peptides</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="eng">Nontraumatic, simple, and reproducible procedures for the introduction of nonpermeant molecules into adherent mammalian cells byin situ electroporation are described. Ctells are grown on a glass slide, half of which is coated with electrically conductive, optically transparent, indium-tin oxide. An electric pulse is applied in the presence of the molecules to be introduced, and their effect on the cellular phenotype can be observed. The cells growing on the nonconductive side of the slide do not receive any pulse and serve as controls. Careful adjustment of electric field strength can achieve the introduction of the molecules into essentially 100% of the cells, and this treatment causes no detectable disruption to cellular metabolism. This is applied in the presence of the fluorescent dye, Lucifer yellow, causing its penetration into the cells growing on the conductive half of the slide. The migration of the dye to the nonelectroporated cells growing on the nonconductive area is microscopically observed under fluorescence illumination.</div></front></TEI><mods xsi:schemaLocation="http://www.loc.gov/mods/v3 file:///applis/istex/home/loadistex/home/etc/xsd/mods.xsd" version="3.4" istexId="0976e58c450746347de1838e7edce5dd8f87e824"><titleInfo lang="eng"><title>applications of electroporation of adherent cellsIn Situ, on a partly conductive slide</title></titleInfo><name type="personal"><namePart type="given">Leda H.</namePart><namePart type="family">Raptis</namePart><role><roleTerm type="text">author</roleTerm></role><affiliation>Department of Microbiology and Immunology, Queen’s University, K7L3N6, Kingston, Ontario, Canada</affiliation></name><name type="personal"><namePart type="given">Heather L.</namePart><namePart type="family">Brownell</namePart><role><roleTerm type="text">author</roleTerm></role><affiliation>Department of Microbiology and Immunology, Queen’s University, K7L3N6, Kingston, Ontario, Canada</affiliation></name><name type="personal"><namePart type="given">Stanley K. W.</namePart><namePart type="family">Liu</namePart><role><roleTerm type="text">author</roleTerm></role><affiliation>Department of Microbiology and Immunology, Queen’s University, K7L3N6, Kingston, Ontario, Canada</affiliation></name><name type="personal"><namePart type="given">Kevin L.</namePart><namePart type="family">Firth</namePart><role><roleTerm type="text">author</roleTerm></role><affiliation>Anatomy and Cell Biology, Queen’s University, K7L3N6, Kingston, Ontario, Canada</affiliation></name><name type="personal"><namePart type="given">Leslie W.</namePart><namePart type="family">MacKenzie</namePart><role><roleTerm type="text">author</roleTerm></role><affiliation>Ask Science Products Inc., Kingston, Ont., Canada</affiliation></name><name type="personal"><namePart type="given">Charles D.</namePart><namePart type="family">Stiles</namePart><role><roleTerm type="text">author</roleTerm></role><affiliation>Division of Cellular and Molecular Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA, </affiliation></name><name type="personal"><namePart type="given">John A.</namePart><namePart type="family">Alberta</namePart><role><roleTerm type="text">author</roleTerm></role><affiliation>Division of Cellular and Molecular Biology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA, </affiliation></name><typeOfResource>text</typeOfResource><genre>Protocol</genre><genre>Original Paper</genre><originInfo><publisher>Humana Press, Totowa</publisher><dateValid encoding="w3cdtf">2008-06-24</dateValid><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="eng">Nontraumatic, simple, and reproducible procedures for the introduction of nonpermeant molecules into adherent mammalian cells byin situ electroporation are described. Ctells are grown on a glass slide, half of which is coated with electrically conductive, optically transparent, indium-tin oxide. An electric pulse is applied in the presence of the molecules to be introduced, and their effect on the cellular phenotype can be observed. The cells growing on the nonconductive side of the slide do not receive any pulse and serve as controls. Careful adjustment of electric field strength can achieve the introduction of the molecules into essentially 100% of the cells, and this treatment causes no detectable disruption to cellular metabolism. This is applied in the presence of the fluorescent dye, Lucifer yellow, causing its penetration into the cells growing on the conductive half of the slide. The migration of the dye to the nonelectroporated cells growing on the nonconductive area is microscopically observed under fluorescence illumination.</abstract><subject lang="eng"><genre>Index Entries</genre><topic>Electroporation</topic><topic>adherent cells</topic><topic>indium-tin oxide</topic><topic>peptides</topic><topic>intercellular communication</topic><topic>gap junctions</topic></subject><relatedItem type="series"><titleInfo type="abbreviated"><title>Mol Biotechnol</title></titleInfo><titleInfo><title>Molecular Biotechnology</title><partNumber>Year: 1995</partNumber><partNumber>Volume: 4</partNumber><partNumber>Number: 2</partNumber></titleInfo><genre>Archive Journal</genre><originInfo><dateIssued encoding="w3cdtf">1995-10-01</dateIssued><copyrightDate encoding="w3cdtf">1995</copyrightDate></originInfo><subject usage="primary"><topic>Chemistry</topic><topic>Biotechnology</topic><topic>Biochemistry, general</topic><topic>Cell Biology</topic><topic>Protein Science</topic><topic>Biological Techniques</topic><topic>Human Genetics</topic></subject><identifier type="issn">1073-6085</identifier><identifier type="issn">Electronic: 1559-0305</identifier><identifier type="matrixNumber">12033</identifier><identifier type="local">IssueArticleCount: 16</identifier><recordInfo><recordOrigin>Humana Press Inc., 1995</recordOrigin></recordInfo></relatedItem><identifier type="doi">10.1007/BF02921607</identifier><identifier type="matrixNumber">Art3</identifier><identifier type="local">BF02921607</identifier><accessCondition type="use and reproduction">MetadataGrant: OpenAccess</accessCondition><accessCondition type="use and reproduction">AbstractGrant: OpenAccess</accessCondition><accessCondition type="restriction on access">BodyPDFGrant: Restricted</accessCondition><accessCondition type="restriction on access">BodyHTMLGrant: Restricted</accessCondition><accessCondition type="restriction on access">BibliographyGrant: Restricted</accessCondition><accessCondition type="restriction on access">ESMGrant: Restricted</accessCondition><part><extent unit="pages"><start>129</start><end>138</end></extent></part><recordInfo><recordOrigin>Humana Press Inc., 1995</recordOrigin><recordIdentifier>12033_1995_Article_BF02921607.pdf</recordIdentifier></recordInfo></mods></record>Pour manipuler ce document sous Unix (Dilib)
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